T5130

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Improved stability and electrophoretic properties of preformed fluorescent cationic dye-DNA complexes in a taps-tetrapentylammonium buffer in agarose slab gels.

Z Zeng et al.

Analytical biochemistry, 252(1), 110-114 (1997-11-05)

High-resolution capillary electrophoresis sizing of preformed complexes of bis-intercalating fluorescent dyes with double-stranded DNA has been demonstrated using hydroxyethylcellulose and 3-[tris-(hydroxymethyl) methylamino]-1-propanesulfonic acid-tetrapentylammonium (Taps-NPe+4) buffers (S. M. Clark and R. A. Mathies, Anal. Chem. 69, 1355-1363, 1997). Such capillary electrophoresis

Producing glucose 6-phosphate from cellulosic biomass: structural insights into levoglucosan bioconversion.

John-Paul Bacik et al.

The Journal of biological chemistry, 290(44), 26638-26648 (2015-09-12)

The most abundant carbohydrate product of cellulosic biomass pyrolysis is the anhydrosugar levoglucosan (1,6-anhydro-β-d-glucopyranose), which can be converted to glucose 6-phosphate by levoglucosan kinase (LGK). In addition to the canonical kinase phosphotransfer reaction, the conversion requires cleavage of the 1,6-anhydro

The [NiFe]-Hydrogenase of Pyrococcus furiosus Exhibits a New Type of Oxygen Tolerance.

Patrick Kwan et al.

Journal of the American Chemical Society, 137(42), 13556-13565 (2015-10-06)

We report the first direct electrochemical characterization of the impact of oxygen on the hydrogen oxidation activity of an oxygen-tolerant, group 3, soluble [NiFe]-hydrogenase: hydrogenase I from Pyrococcus furiosus (PfSHI), which grows optimally near 100 °C. Chronoamperometric experiments were used

Formation of concentration gradient and its application to DNA capillary electrophoresis.

D Liang et al.

Electrophoresis, 21(17), 3600-3608 (2001-03-29)

A new method to introduce the concentration gradient into the capillary has been developed and its application to DNA capillary electrophoresis is presented. The concentration gradient produced by mixing 5% w/v polyacrylamide-co-poly(N-dimethylacrylamide) (PAM-co-PDMA) solution and 1 x Tris/N-tris(hydroxymethyl)methyl-3-amino-propanesulfonic acid/EDTA (TT)

Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration.

Myra Noemi Chávez et al.

Biomaterials, 75, 25-36 (2015-10-17)

The use of artificial tissues in regenerative medicine is limited due to hypoxia. As a strategy to overcome this drawback, we have shown that photosynthetic biomaterials can produce and provide oxygen independently of blood perfusion by generating chimeric animal-plant tissues

CoBATCH for High-Throughput Single-Cell Epigenomic Profiling.

Qianhao Wang et al.

Molecular cell, 76(1), 206-216 (2019-09-01)

An efficient, generalizable method for genome-wide mapping of single-cell histone modifications or chromatin-binding proteins is lacking. Here, we develop CoBATCH, combinatorial barcoding and targeted chromatin release, for single-cell profiling of genomic distribution of chromatin-binding proteins in cell culture and tissue.

Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons.

Seung-ick Oh et al.

TheScientificWorldJournal, 2014, 957548-957548 (2014-07-06)

Somatic cells were directly converted to functional neurons through the use of a combination of transcription factors, including Ascl1, Brn2, and Myt1l. However, a major limitation is the lack of a reliable source of cell-replacement therapy for neurological diseases. Here

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