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Cyrielle Briand et al.
Scientific reports, 7, 41703-41703 (2017-02-06)
Nitrate content of surface waters results from complex mixing of multiple sources, whose signatures can be modified through N reactions occurring within the different compartments of the whole catchment. Despite this complexity, the determination of nitrate origin is the first
R B Miller
Journal of pharmaceutical and biomedical analysis, 9(10-12), 953-958 (1991-01-01)
A stereospecific method for the analysis of propranolol and 4-hydroxypropranolol in human plasma employing fluorescence detection has been developed using the homochiral derivatizing agent 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate (TAGIT). The use of fluorescence detection provided enhanced sensitivity and cleaner chromatograms for the
Bushra Saeed et al.
BMC genomics, 17, 98-98 (2016-02-06)
High potential of Morus laevigata and Morus serrata has been proposed in the breeding programs for Morus sp. However, due to the lack of dense molecular markers this goal is still in its nascent stage and not yet realized. We
A Mozayani et al.
Journal of analytical toxicology, 19(6), 519-521 (1995-10-01)
The distribution of the racemic and the enantiomeric content of (+/-)-metoprolol was compared after ingestion of a massive fatal overdose of the racemic drug. Postmortem concentrations of the racemate in different tissues were assayed by gas chromatography after derivatization with
Reversed-phase liquid chromatographic resolution of amino acid enantiomers by derivatization with 2, 3, 4, 6-tetra-O-acetyl-?-D-glucopyranosyl isothiocyanate.
Nimura, Noriyuki, Haruo Ogura, and Toshio Kinoshita.
Journal of Chromatography A, 202.3, 375-379 (1980)
I C Uzoma et al.
Nigerian journal of clinical practice, 22(1), 51-55 (2019-01-23)
The presence of BCR-ABL1 fusion gene resulting from a t(9; 22) reciprocal chromosome translocation is the molecular hallmark of chronic myeloid leukemia (CML). In the diagnosis and treatment of CML, peripheral blood or bone marrow samples are usually taken for
S Chakravorty et al.
FEMS microbiology letters, 205(1), 113-117 (2001-12-01)
Nucleic acid amplification technologies offer great promise for the rapid, sensitive and specific diagnosis of tuberculosis. However, the isolation of inhibitor-free DNA from biological specimens is a bottleneck of the PCR assay. Here we describe a simple method for the
S T Wu et al.
Journal of chromatography. B, Biomedical sciences and applications, 692(1), 133-140 (1997-04-25)
A stereoselective reversed-phase HPLC assay to quantify S-(-) and R-(+) enantiomers of propranolol and 4-hydroxypropranolol in human plasma was developed. The method involved liquid-liquid extraction for sample clean-up and employed 2,3,4,6-tetra-O-acetyl-beta-glucopyranosyl isothiocyanate as a pre-column chiral derivatization reagent. The internal
Agne Malki et al.
Journal of leukocyte biology, 97(3), 533-545 (2015-01-28)
Our cellular immune system has to cope constantly with foodborne substances that enter the bloodstream postprandially. Here, they may activate leukocytes via specific but yet mostly unknown receptors. Ectopic RNA expression out of gene families of chemosensory receptors, i.e., the
Stephen R Welch et al.
Journal of clinical microbiology, 58(11) (2020-08-26)
The COVID-19 pandemic has necessitated a multifaceted rapid response by the scientific community, bringing researchers, health officials, and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with
Hemant Suryawanshi et al.
Science advances, 4(10), eaau4788-eaau4788 (2018-11-08)
The placenta and decidua interact dynamically to enable embryonic and fetal development. Here, we report single-cell RNA sequencing of 14,341 and 6754 cells from first-trimester human placental villous and decidual tissues, respectively. Bioinformatic analysis identified major cell types, many known
Kevan M Yamahara et al.
PloS one, 15(4), e0232054-e0232054 (2020-05-01)
Microbial source tracking and a mass balance approach were used to identify sources of fecal indicator bacteria (FIB) in the Hanalei River, Kaua'i, Hawai'i. Historically, concentrations enterococci and Clostridium perfringens were significantly higher during storm flows compared to non-storm flows
[Quantitative determination of optical isomers of pindolol and propranolol using a GITC reagent].
M Kushiya et al.
Eisei Shikenjo hokoku. Bulletin of National Institute of Hygienic Sciences, (104)(104), 103-108 (1986-01-01)
L He et al.
Biomedical chromatography : BMC, 6(6), 291-294 (1992-11-01)
A high performance liquid chromatographic method was developed for the simultaneous assay of R(-)- and S(+)-albuterol in human serum. The assay involves solid phase extraction as a sample clean-up step and derivatization of racemic albuterol to its diastereomeric thioureas with
L A Ovchinnikova et al.
Bulletin of experimental biology and medicine, 169(3), 383-387 (2020-08-05)
We optimized a method for the preparation and purification of self-assembled protein nanocontainers EPN, the latest achievement in protein engineering. These nanocontainers are highly stable and provide the possibility of highly specific loading of a protein therapeutic drug. The described
Sonja Hess
Methods in molecular biology (Clifton, N.J.), 828, 63-75 (2011-11-30)
The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences, including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in
Wenkai Hui et al.
Journal of separation science, 39(17), 3302-3310 (2016-07-09)
We report the development and validation of a stability-indicating reversed-phase high-performance liquid chromatography method with precolumn derivatization for the separation and identification of the impurities of ripasudil hydrochloride hydrate, a novel protein kinase inhibitor. 2,3,4,6-Tetra-O-acetyl-β-d-glucopyranosyl isothiocyanate was chosen as the
Kinoshita, T., et al.
Journal of Chromatography A, 202, 375-375 (1980)
Maria Walczak et al.
Chirality, 24(8), 591-599 (2012-06-21)
A sensitive and stereospecific liquid chromatography-tandem mass spectrometry method for the quantitative determination of TWo8 enantiomers ((2RS)-1-(7-methoxy-1H-indol-4-yloxy)-3-(2-(2-methoxyphenoxy)ethylamino)-propan-2-ol) was developed and validated in rat serum and some tissues. Racemic TWo8 is a new chemical entity, and it has been shown to
Bushra Saeed et al.
Plant physiology and biochemistry : PPB, 111, 174-178 (2016-12-13)
Early Responsive to Dehydration (ERD) genes are described as rapid response mediators of dehydration stress. Recently, ERD15 has emerged as a novel stress induced transcription factor which might be involved in mediating distinct stress responses in plants. In order to
G Neugebauer et al.
European journal of clinical pharmacology, 38 Suppl 2, S108-S111 (1990-01-01)
The racemic compound carvedilol is a multiple-action oral antihypertensive drug that exhibits both vasodilator and non-selective beta-adrenergic blocking activities. The effects of the levorotatory S-enantiomer [S(-)-CARV] are vasodilatation and beta-blockade. The R(+)-enantiomer [R(+)-CARV] is a pure vasodilating agent. Quantitative determination
T Kinoshita
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 118(2), 31-50 (1998-03-26)
Highly effective separation and highly sensitive detection reagents for clinical chemistry and biochemistry were developed and their applications were investigated. The sensitive detection of carboxylic acids was accomplished using 9-anthryldiazomethane (ADAM) which gave intensely fluorescent derivatives from carboxylic acids without
K H Kim et al.
Archives of pharmacal research, 23(6), 568-573 (2001-01-13)
A reversed-phase high-performance liquid chromatographic method was developed to determine the optical purity of bevantolol enantiomers. (S)-(-)-Menthyl chloroformate((-)-MCF), (S)-(-)-alpha-methylbenzyl isocyanate((-)-MBIC) and 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate(GITC), which can react with the secondary amine group of bevantolol were investigated as chiral derivatization reagents. Among
Christopher K Raymond et al.
PloS one, 15(6), e0231854-e0231854 (2020-06-02)
UltraPrep is an open-source, two-step method for purification of cell-free DNA that entails extraction of total DNA followed by size-selective enrichment of the smaller fragments that are characteristic of DNA originating from fragmentation between nucleosome. The advantages of the two
Zhiqiong Chen et al.
Journal of separation science, 28(2), 193-196 (2005-03-10)
Chiral separation of racemic mixtures is of the greatest importance to the pharmaceutical industry, as the isomers of a given racemate may exhibit substantially different pharmacological effects, not to mention possibly differing toxicity behaviour. A novel chiral separation method is
Boris Pastorino et al.
Viruses, 12(6) (2020-06-12)
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses
T Shimizu et al.
Journal of chromatography. B, Biomedical applications, 674(1), 77-83 (1995-12-01)
A simple and sensitive high-performance liquid chromatographic (HPLC) method for the determination of (+)-(S)-sotalol and (-)-(R)-sotalol in biological fluids was established. Following extraction with isopropyl alcohol from biological samples on a Sep-Pak C18 cartridge, the eluent was derivatized with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl
Z Y Yang et al.
Chirality, 1(1), 92-93 (1989-01-01)
Epinephrine enantiomers were derived into diastereoisomers with the chiral reagent 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylisothiocyanate. The resolution was carried out on a C18 column. The Rs between (-)-R- and (+)-S-isomers was 2.3. The retention time could be changed by adding a proper amount of
Zahra Jahanshiri et al.
World journal of microbiology & biotechnology, 31(7), 1071-1078 (2015-04-22)
Aflatoxin contamination of grains and agro-products is a serious food safety issue and a significant economic concern worldwide. In the present study, the effects of eugenol on Aspergillus parasiticus growth and aflatoxin production were studied in relation to the expression
Michael T Furlong et al.
Journal of pharmaceutical and biomedical analysis, 70, 574-579 (2012-06-20)
Carvedilol is widely prescribed for the treatment of hypertension, heart failure and left ventricular dysfunction following myocardial infarction. A sensitive and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay was developed and validated to enable reliable and efficient bioanalysis of the
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