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David P Eisenberg et al.
Cryobiology, 65(2), 117-125 (2012-05-15)
This study explores physical effects associated with the application of cryopreservation via vitrification using a class of compounds which are defined here as synthetic ice modulators (SIMs). The general classification of SIMs includes molecules that modulate ice nucleation and growth
J P Casazza et al.
Alcohol and alcoholism (Oxford, Oxfordshire). Supplement, 1, 607-609 (1987-01-01)
Plasma 1,2-propanediol, D,L,-2,3-butanediol and 2,3-meso-butanediol were measured in 29 patients with biopsy proven alcoholic cirrhosis and 10 control subjects, none of whom had measurable blood alcohol levels. None of these compounds were present in control subjects at level above 5
Jong Myoung Park et al.
Microbial cell factories, 12, 20-20 (2013-02-26)
Klebsiella oxytoca, a Gram-negative, rod-shaped, and facultative anaerobic bacterium, is one of the most promising 2,3-butanediol (2,3-BD) producers. In order to improve the metabolic performance of K. oxytoca as an efficient biofactory, it is necessary to assess its metabolic characteristics
Meng Wang et al.
Biotechnology letters, 34(10), 1877-1885 (2012-06-21)
Acetoin is widely used in food and other industries. A bdhA and acoA double-knockout strain of Bacillus subtilis produced acetoin at 0.72 mol/mol, a 16.4 % increased compared to the wild type. Subsequent overexpression of the alsSD operon enhanced the
Sung-Geun Jung et al.
Applied microbiology and biotechnology, 97(5), 1997-2007 (2012-07-27)
Klebsiella species are the most extensively studied among a number of 2,3-butanediol (2,3-BDO)-producing microorganisms. The ability to metabolize a wide variety of substrates together with the ease of cultivation made this microorganisms particularly promising for the application in industrial-scale production
Xiaolin Shen et al.
Journal of industrial microbiology & biotechnology, 39(11), 1725-1729 (2012-07-27)
This work describes the production of (R,R)-2,3-butanediol in Escherichia coli using glycerol by metabolic engineering approaches. The introduction of a synthetic pathway converting pyruvate to (R,R)-2,3-butanediol into wild-type E. coli strain BW25113 led to the production of (R,R)-2,3-butanediol at a
Bo Zhang et al.
Applied microbiology and biotechnology, 99(2), 885-896 (2015-01-27)
Efficient utilization of xylose by bacteria is essential for production of fuels and chemicals from lignocellulosic biomass. In this study, Bacillus subtilis 168 was subjected to laboratory adaptive evolution, and a mutant E72, which could grow on xylose with a
E Celińska et al.
Biotechnology advances, 27(6), 715-725 (2009-05-16)
Biotechnological production of 2,3-butanediol (hereafter referred to as 2,3-BD) from wastes and excessive biomass is a promising and attractive alternative for traditional chemical synthesis. In the face of scarcity of fossil fuel supplies the bio-based process is receiving a significant
Shuji Ohno et al.
Steroids, 70(9), 645-651 (2005-05-19)
We previously reported that tributyltin chloride (TBT) and triphenyltin chloride (TPT) powerfully suppressed human chorionic gonadotropin- and 8-bromo-cAMP-stimulated testosterone production in pig Leydig cells at concentrations that were not cytotoxic [Nakajima Y, Sato Q, Ohno S, Nakajin S. Organotin compounds
M J Syu
Applied microbiology and biotechnology, 55(1), 10-18 (2001-03-10)
2,3-Butanediol (2,3-BDL), which is very important for a variety of chemical feedstocks and liquid fuels, can be derived from the bioconversion of natural resources. One of its well known applications is the formation of methyl ethyl ketone, by dehydration, which
J R Thornton et al.
Gut, 30(10), 1392-1395 (1989-10-01)
Plasma methionine enkephalin is increased in liver disease and may contribute to some of the clinical manifestations of hepatic failure. To determine if another 'small' opioid peptide is increased in the plasma of patients with liver disease, leucine enkephalin was
Anupama Shrivastav et al.
Journal of microbiology and biotechnology, 23(7), 885-896 (2013-06-04)
2,3-Butanediol (2,3-BDO) has immense industrial applications. Recently, microbial fermentation has emerged as an alternative way to produce this industrially important chemical. Although 2,3-BDO is produced by several microorganisms, the Klebsiella genera has an excellent production compared with other 2,3-BDO-producing microorganisms.
Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria.
Nicole E Nozzi et al.
ACS synthetic biology, 4(11), 1197-1204 (2015-05-15)
Cyanobacteria have gained popularity among the metabolic engineering community as a tractable photosynthetic host for renewable chemical production. However, though a number of successfully engineered production systems have been reported, long-term genetic stability remains an issue for cyanobacterial systems. The
A K Batta et al.
Journal of lipid research, 30(12), 1953-1962 (1989-12-01)
We have studied the effect of ursodeoxycholic acid on the serum and urinary bile acids in seven patients with moderate to severe primary biliary cirrhosis. Bile acids were characterized by gas-liquid chromatography-mass spectrometry and quantified by capillary gas-liquid chromatography. Serum
J Szebeni et al.
Alcoholism, clinical and experimental research, 10(6), 647-650 (1986-12-01)
The level of conjugated dienes (CD) in the serum, conjugated linoleic acid isomer in the phospholipid fraction [18:2(9, 11)], and the fatty acid profile in both the serum and in the separated lipid classes were analyzed in current and previous
M K Sachs et al.
American journal of clinical pathology, 96(3), 381-384 (1991-09-01)
A patient with squamous cell carcinoma of the lung and a serum rheumatoid factor (RF) of 1:1,280 had a positive cerebrospinal fluid (CSF) latex agglutination test (LAT) for cryptococcal antigen, in culture-negative, India-ink-negative CSF. Pretreatment of the sample of CSF
Gen Lin Zhang et al.
Biotechnology letters, 34(8), 1519-1523 (2012-05-02)
The budC gene encoding the meso-2,3-BDH from Klebsiella pneumoniae XJ-Li was expressed in E. coli BL21 (DE3) pLys. Hypothetical amino acid sequence alignments revealed that the enzyme belongs to the short chain dehydrogenase/reductase family. After purification and refolding, the recombinant
Patricia Hermand et al.
The Journal of biological chemistry, 278(7), 4892-4898 (2002-12-13)
ICAM-4 (LW blood group glycoprotein) is an erythroid-specific membrane component that belongs to the family of intercellular adhesion molecules and interacts in vitro with different members of the integrin family, suggesting a potential role in adhesion or cell interaction events
Yunju Jin et al.
Neuroscience letters, 369(3), 250-255 (2004-10-07)
TRPV1, a receptor for capsaicin, plays a key role in mediating thermal and inflammatory pain. Because the modulation of ion channels by the cellular redox state is a significant determinant of channel function, we investigated the effects of sulfhydryl modification
Yuansheng Xue et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 27(12), 1742-1748 (2012-04-18)
The production of 2, 3-butanediol and succinic acid by a moderate halophile under anaerobic condition was investigated. This halophile, termed Salinivibrio YS, was isolated from the solid samples collected from Aydingkol Lake. Based on the single factor experiment, the parameters
Hyun-Dong Shin et al.
Bioresource technology, 118, 367-373 (2012-06-19)
Escherichia coli has been engineered to produce a variety of biofuel and biorefinery products. However, it can only produce these products from simple sugars, requiring large amounts of enzymes to depolymerize cellulose into monomer sugars. Engineering E. coli to directly
Liqun Jiang et al.
Bioresource technology, 196, 194-199 (2015-08-05)
In this study, the effects of glycerol pretreatment on subsequent glycerol fermentation and biomass fast pyrolysis were investigated. The liquid fraction from the pretreatment process was evaluated to be feasible for fermentation by Paenibacillus polymyxa and could be an economic
C J McClain et al.
Gut, 21(4), 318-323 (1980-04-01)
Blood methanethiol and ammonia concentrations were measured in 16 healty volunteers, 52 consecutive alcoholic cirrhotics without overt hepatic encephalopathy (HE), and 42 consecutive patients with alcoholic liver disease and overt HE. The mean concentration of blood methanethiol was significantly greater
John W K Oliver et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(4), 1249-1254 (2013-01-09)
Conversion of CO(2) for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically
Jinshan Li et al.
Applied microbiology and biotechnology, 97(2), 585-597 (2012-08-07)
Paenibacillus polymyxa can produce the (R,R)-stereoisomer of 2,3-butanediol (2,3-BDL) which is industrially very useful. Two important factors affecting (R,R)-BDL production by P. polymyxa ATCC 12321, medium composition, and addition of acetic acid to the culture were investigated in this study
Sangjun Jeon et al.
Journal of bioscience and bioengineering, 117(4), 464-470 (2013-10-23)
This study presents a new and effective downstream process to recover 2,3-butanediol (2,3-BD) from fermentation broth which is produced by a recombinant Klebsiella pneumoniae strain. The ldhA-deficient K. pneumoniae strain yielded about 90 g/L of 2,3-BD, along with a number
Eduard Calam et al.
Chemico-biological interactions, 202(1-3), 195-203 (2013-01-09)
The α-hydroxy ketones are used as building blocks for compounds of pharmaceutical interest (such as antidepressants, HIV-protease inhibitors and antitumorals). They can be obtained by the action of enzymes or whole cells on selected substrates, such as diketones. We have
Mingshou Lu et al.
Journal of microbiology and biotechnology, 22(5), 659-667 (2012-05-09)
2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway
Zhi-Long Xiu et al.
Applied microbiology and biotechnology, 78(6), 917-926 (2008-03-06)
1,3-Propanediol and 2,3-butanediol are two promising chemicals which have a wide range of applications and can be biologically produced. The separation of these diols from fermentation broth makes more than 50% of the total costs in their microbial production. This
Frank Plaisant et al.
Free radical biology & medicine, 34(7), 862-872 (2003-03-26)
The pathophysiology of brain lesions associated with cerebral palsy is multifactorial and likely involves excess release of glutamate and excess production of free radicals, among other factors. Theoretically, antioxidants could limit the severity of these brain lesions. Peroxiredoxins are a
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