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关键词:'X114'
显示 1-30 共 205 条结果 关于 "X114" 范围 论文
Marta Hryniewicka et al.
Journal of pharmaceutical and biomedical analysis, 106, 129-135 (2014-12-10)
This work illustrates the development of new procedures for the isolation and preconcentration of fluvastatin (FLU) from aqueous solutions. Micellar extraction (ME) combined with high performance liquid chromatography (HPLC-UV) has been successfully applied for this purpose. It was found that
Mateusz Dobrowolski et al.
Development (Cambridge, England), 147(2) (2020-01-15)
Oligodendrocyte development is tightly controlled by extrinsic signals; however, mechanisms that modulate cellular responses to these factors remain unclear. Six-transmembrane glycerophosphodiester phosphodiesterases (GDEs) are emerging as central regulators of cellular differentiation via their ability to shed glycosylphosphatidylinositol (GPI)-anchored proteins from
Te-Sheng Lien et al.
Frontiers in immunology, 12, 617251-617251 (2021-03-16)
Typically occurring during secondary dengue virus (DENV) infections, dengue hemorrhagic fever (DHF) causes abnormal immune responses, as well as endothelial vascular dysfunction, for which the responsible viral factor remains unclear. During peak viremia, the plasma levels of virion-associated envelope protein
Anita K Ho et al.
BMC biology, 14(1), 109-109 (2016-12-09)
Mutations in Lipopolysaccharide-induced tumour necrosis factor-α factor (LITAF) cause the autosomal dominant inherited peripheral neuropathy, Charcot-Marie-Tooth disease type 1C (CMT1C). LITAF encodes a 17 kDa protein containing an N-terminal proline-rich region followed by an evolutionarily-conserved C-terminal 'LITAF domain', which contains all
Patrick M Siegel et al.
Basic research in cardiology, 116(1), 17-17 (2021-03-16)
The monocyte β2-integrin Mac-1 is crucial for leukocyte-endothelium interaction, rendering it an attractive therapeutic target for acute and chronic inflammation. Using phage display, a Designed-Ankyrin-Repeat-Protein (DARPin) was selected as a novel binding protein targeting and blocking the αM I-domain, an
José Martín Rosas Castor et al.
Food chemistry, 204, 475-482 (2016-03-19)
A simple, inexpensive and rapid method was proposed for the determination of bioaccessible arsenic in corn and rice samples using an in vitro bioaccessibility assay. The method was based on the preconcentration of arsenic by cloud point extraction (CPE) using
Susanna Raho et al.
Nature metabolism, 2(12), 1373-1381 (2020-11-25)
The oncogenic KRAS mutation has a critical role in the initiation of human pancreatic ductal adenocarcinoma (PDAC) since it rewires glutamine metabolism to increase reduced nicotinamide adenine dinucleotide phosphate (NADPH) production, balancing cellular redox homeostasis with macromolecular synthesis1,2. Mitochondrial glutamine-derived
Wael I Mortada et al.
Biological trace element research, 168(2), 303-310 (2015-05-23)
Cadmium (Cd) level in blood and urine of soldering iron workers (n=49) and 41 matched healthy controls has been assessed. Cloud point extraction was employed for preconcentration of Cd. The Cd ions formed hydrophobic complex with 9,10-phenanthraquinone monoethyl thiosemicarbazone that
Dmitrii A Komissarenko et al.
Materials (Basel, Switzerland), 11(12) (2018-11-25)
The present study demonstrates the possibility of fabricating zirconia parts with a complex shape and internal architecture using a low-cost stereolithography-based technique. One of the critical steps in ceramics stereolithography is the preparation of a photo-curable slurry with properties that
Efseveia Frakolaki et al.
Cells, 8(8) (2019-08-08)
l-dopa decarboxylase (DDC) that catalyzes the biosynthesis of bioactive amines, such as dopamine and serotonin, is expressed in the nervous system and peripheral tissues, including the liver, where its physiological role remains unknown. Recently, we reported a physical and functional
Masumi Iijima et al.
Biosensors & bioelectronics, 150, 111860-111860 (2019-11-19)
The orientation and density of biosensing molecules on sensor chip should be precisely controlled to improve sensitivity and ligand-binding capacity. We previously developed a ~30-nm bio-nanocapsule (ZZ-BNC), consisting of the hepatitis B virus envelope L protein fused with the tandem
Trícia Maria Ferreira de Sousa Oliveira et al.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 24(2), 220-222 (2015-07-15)
The relevance of the dog as a source of visceral leishmaniasis infection is known, but the role of cats as reservoir hosts for leishmaniasis is not yet fully clear. This study assessed the efficacy of conjunctival swab PCR (CS-PCR) in
K Moffitt et al.
Clinical and vaccine immunology : CVI, 22(8), 909-916 (2015-06-05)
A pneumococcal whole-cell vaccine (WCV) confers T(H)17-mediated immunogenicity and reduces nasopharyngeal (NP) carriage in mice. Activation of Toll-like receptor 2 (TLR2) has been shown to be important for generating T(H)17 responses, and several lipidated pneumococcal proteins have TLR2-activating properties. Here
H Yang et al.
Journal of internal medicine, 282(1), 76-93 (2017-05-04)
Extra-corpuscular haemoglobin is an endogenous factor enhancing inflammatory tissue damage, a process counteracted by the haemoglobin-binding plasma protein haptoglobin composed of alpha and beta subunits connected by disulfide bridges. Recent studies established that haptoglobin also binds and sequesters another pro-inflammatory
Jia-Ni Xiao et al.
Frontiers in cellular and infection microbiology, 7, 495-495 (2017-12-19)
Tuberculosis (TB) remains one of the most severe infectious diseases. It is still of paramount importance to establish more accurate, rapid, and efficient diagnostic methods. Since infection with
Stephen J Savarino et al.
The Journal of infectious diseases, 220(3), 505-513 (2019-03-22)
Enterotoxigenic Escherichia coli (ETEC) commonly cause diarrhea in children living in developing countries and in travelers to those regions. ETEC are characterized by colonization factors (CFs) that mediate intestinal adherence. We assessed if bovine colostral IgG (bIgG) antibodies against a
Medea Neek et al.
Biomaterials, 156, 194-203 (2017-12-05)
Nanoparticles have attracted considerable interest as cancer vaccine delivery vehicles for inducing sufficient CD8
John E Bradley et al.
Laboratory investigation; a journal of technical methods and pathology, 93(3), 365-374 (2013-01-30)
Thymocyte differentiation antigen-1 (Thy-1) is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein expressed on numerous cell types, which regulates signals affecting cell adhesion, migration, differentiation, and survival. In addition, Thy-1 has been detected in the serum, cerebral spinal fluid, wound fluid
Ting Yang et al.
Scientific reports, 5, 16733-16733 (2015-11-18)
The distinct roles of protein isoforms in cancer are becoming increasingly evident. FRα and FRβ, two major isoforms of the folate receptor family, generally have different cellular distribution and tissue specificity. However, the presence of FRβ in breast tumors, where
David Y Pereira et al.
Analytica chimica acta, 882, 83-89 (2015-06-05)
The lateral-flow immunoassay (LFA) is an inexpensive and rapid paper-based assay that can potentially detect infectious disease biomarkers in resource-poor settings. Despite its many advantages that make it suitable for point-of-care diagnosis, LFA is limited by its inferior sensitivity relative
Y Aida et al.
Journal of immunological methods, 132(2), 191-195 (1990-09-14)
Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37 degrees C, whereupon two phases
Sanja Škulj et al.
International journal of molecular sciences, 22(3) (2021-02-04)
Molecular dynamics (MD) simulations of uncoupling proteins (UCP), a class of transmembrane proteins relevant for proton transport across inner mitochondrial membranes, represent a complicated task due to the lack of available structural data. In this work, we use a combination
M Cotten et al.
Gene therapy, 1(4), 239-246 (1994-07-01)
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)
Ryan J Schulze et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(51), 32443-32452 (2020-12-09)
Hepatocytes metabolize energy-rich cytoplasmic lipid droplets (LDs) in the lysosome-directed process of autophagy. An organelle-selective form of this process (macrolipophagy) results in the engulfment of LDs within double-membrane delimited structures (autophagosomes) before lysosomal fusion. Whether this is an exclusive autophagic
Flemming Jessen et al.
Analytical biochemistry, 485, 11-17 (2015-06-17)
A simple and reproducible procedure for enrichment of a plasma protein subfraction suitable for two-dimensional polyacrylamide gel electrophoresis (2DE) was developed, using a Triton X-114-based cloud point extraction (CPE). Appropriate conditions for such a CPE procedure were found by SDS-PAGE
Nam Joong Kim et al.
Molecular immunology, 65(1), 17-24 (2015-01-21)
Staphylococcus aureus is a Gram-positive bacterium that causes inflammation at infection sites by inducing various inflammatory mediators such as nitric oxide (NO). To identify the staphylococcal virulence factors contributing to NO production, we compared the ability of ethanol-killed wild-type S.
Jamshed Ali et al.
Food chemistry, 344, 128706-128706 (2020-12-04)
A green vortex assisted based liquid-liquid microextraction (VA-LLME) method was developed for preconcentration of selenium. Ammonium pyrrolidine dithiocarbamate (APDC) was used to form a hydrophobic complex with selenium in natural water, agricultural soil and food samples by GFAAS. Whereas Triton
Bijun Zhu et al.
Experimental eye research, 162, 73-78 (2017-07-20)
MiRNAs are expressed in a tissue-specific fashion in the eyes and changes in miRNAs levels in aqueous humor (AH) may reflect the function of the eye and eye disease. Due to the low concentration of total miRNA in human aqueous
Louisa Warryn et al.
PLoS neglected tropical diseases, 14(6), e0008357-e0008357 (2020-06-27)
Mycolactones, macrolide cytotoxins, are key virulence factors of Mycobacterium ulcerans, the etiological agent of the chronic necrotizing skin disease Buruli ulcer. There is urgent need for a simple point-of-care laboratory test for Buruli ulcer and mycolactone represents a promising target
R Lappe et al.
Natural product research, 26(23), 2259-2265 (2012-01-21)
Cerein 8A is an antimicrobial peptide with potential application against food spoilage and pathogenic bacteria. The partitioning of cerein 8A was investigated in two liquid-liquid extraction systems that are considered promising for bioseparation and purification purposes. Aqueous two-phase systems (ATPSs)
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