Application
硫酸鱼精蛋白用于：
- 将慢病毒转导进人胚胎293T细胞
- 将 shRNA转导入间充质干细胞/骨髓基质细胞（MSC）
- 将 慢病毒Sca1-OCT4质粒转导入人脐血造血祖细胞CD34+ 细胞
Biochem/physiol Actions
碱性鱼精蛋白与带负电荷的肝素结合。在心血管外科手术中使用鱼精蛋白有时会引发过敏反应。
鱼精蛋白有助于使用阳离子脂质体直接转染微生物 细胞和人类细胞。鱼精蛋白能够有效中和肝素的抗凝作用。
General description
硫酸鱼精蛋白是阳离子多肽，从鲑鱼精子中分离得到，分子量5 kDa。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation
Ainle FN, et al.
Blood, 114(8), 1658-1665 (2009)
Moosung Lee et al.
eLife, 9 (2020-12-18)
The immunological synapse (IS) is a cell-cell junction between a T cell and a professional antigen-presenting cell. Since the IS formation is a critical step for the initiation of an antigen-specific immune response, various live-cell imaging techniques, most of which
F L Sorgi et al.
Gene therapy, 4(9), 961-968 (1998-02-12)
A polycationic peptide, protamine sulfate, USP, has been shown to be able to condense plasmid DNA efficiently for delivery into several different types of cells in vitro by several different types of cationic liposomes. The monovalent cationic liposomal formulations (DC-Chol
Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs
Hematopoietic Differentiation of Human Pluripotent Stem Cells, 91-101 (2015)
Meagan R Rollins et al.
Current protocols in immunology, 129(1), e97-e97 (2020-05-21)
T lymphocytes are capable of specific recognition and elimination of target cells. Physiological antigen recognition is mediated by the T cell receptor (TCR), which is an alpha beta heterodimer comprising the products of randomly rearranged V, D, and J genes.
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