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H1256

Sigma-Aldrich

3-(4-Hydroxyphenyl)propionic acid N-hydroxysuccinimide ester

~90%

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Synonym(s):
N-Succinimidyl 3-(4-hydroxyphenyl)propionate, N-[(4-Hydroxyhydrocinnamoyl)oxy]succinimide, Bolton-Hunter reagent precursor, Rudinger reagent, SHPP
Empirical Formula (Hill Notation):
C13H13NO5
CAS Number:
Molecular Weight:
263.25
Beilstein:
1545011
EC Number:
MDL number:
PubChem Substance ID:

Assay

~90%

reaction suitability

reagent type: linker

mp

132-133 °C (lit.)

application(s)

peptide synthesis

storage temp.

−20°C

SMILES string

Oc1ccc(CCC(=O)ON2C(=O)CCC2=O)cc1

InChI

1S/C13H13NO5/c15-10-4-1-9(2-5-10)3-8-13(18)19-14-11(16)6-7-12(14)17/h1-2,4-5,15H,3,6-8H2

InChI key

KYRUKRFVOACELK-UHFFFAOYSA-N

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Application

3-(4-Hydroxyphenyl)propionic acid N-hydroxysuccinimide ester can be used to prepare 125I-labeled reagents, which are used in binding assay studies of proteins and peptides.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Radioimmunoassay of iodinated gentamicin
Broughton, Alan and Strong, James E
Clinica Chimica Acta; International Journal of Clinical Chemistry, 66(1), 125-129 (1976)
Preparation of 125-I-labeled oligosaccharide derivatives with the aid of 3-(4-hydroxyphenyl) propionic acid N-hydroxysuccinimide ester
Himmelspach K, et al.
Febs Letters, 75(1-2), 154-158 (1977)
P Portolés et al.
Journal of immunological methods, 129(1), 105-109 (1990-05-08)
A method for the vectorial radioiodination of CD4 and other membrane proteins having few or no tyrosine residues in the extracytoplasmic domains is described. Incubation of the cells with sulfosuccinimidyl (hydroxyphenyl) propionate (sulfo-SHPP), a water-soluble derivative of the Bolton-Hunter reagent
E Marshall et al.
Molecular and biochemical parasitology, 15(3), 295-304 (1985-06-01)
The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of
A J Tenner et al.
Journal of immunology (Baltimore, Md. : 1950), 127(2), 648-653 (1981-08-01)
A new procedure for isolating human C1q from serum or plasma is described. The method, that is highly selective, rapid and involves minimal handling, yields fully active, immunoglobulin-free unaggregated C1q. Several different methods of radiolabeling C1q are compared. These include

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