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1.50648

Purospher® STAR RP-18 endcapped (2 µm) HPLC Columns

L × I.D. 100 mm x 2.1 mm Hibar® HR UHPLC Column

Synonym(s):

Purospher® STAR RP-18 endcapped (2µm) Hibar® HR 100-2.1

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
2 μm
Matrix active group:
C18 bonding phase
Pore size:
120 Å pore size
Matrix:
fully porous particle

Key Documents

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Product Name

Purospher® STAR RP-18 endcapped (2 µm) UHPLC Columns, L × I.D. 100 mm x 2.1 mm Hibar® HR UHPLC column

material

stainless steel column

agency

suitable for ASTM® 7968, suitable for ASTM® 7979, suitable for DIN 15968-2010, suitable for EPA 1633, suitable for EPA 38407-42, suitable for EPA 533, suitable for EPA 537.1, suitable for EPA 8327, suitable for EPA ACB B21-02, suitable for EPA ACB B23-05b, suitable for GB 5009.253-2016, suitable for ISO 21675 2019, suitable for ISO 25101, suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

1000 bar pressure, 65 °C max. temp.

technique(s)

LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

2 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

application(s)

PFAS testing

separation technique

reversed phase, supercritical fluid (SFC)

storage temp.

no temp limit

Quality Level

100

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General description

Purospher® STAR RP-18 endcapped, 2 µm UHPLC columns are designed for use in UHPLC Applications and instruments providing full UHPLC resolution and highest separation efficiency′s at maximum pressure of 1000 bar.Purospher® STAR RP-18 endcapped, 2 µm UHPLC columns allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 2 µm Hibar® HR 100-2.1 is a ready to use UHPLC column suitable for connection to any UHPLC system providing a pressure stability of 1000 bar.The column dimension of 100 mm length x 2.1 mm I.D. is the optimal choice for fast UHPLC separation of complex samples allowing significant solvent savings and improved sensitivity.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 280 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 170000
Symmetry (1,2-Benzanthracene): 0.8 - 1.4
Capacity factor (1,2-Benzanthracene): 8.5 - 11.5

Legal Information

ASTM is a registered trademark of American Society for Testing and Materials
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Tanaka Test for C18 Stationary Phase in HPLC Columns

Discover the Tanaka Test, a key protocol for evaluating the performance and selectivity of stationary phases in Reversed Phase HPLC separations.

Analysis of Pesticides and their Metabolite Residues

Pesticide residue analysis of 331 pesticides and 44 metabolites in cabbage samples using Supel™ QuE QuEChERS mixes and Purospher® STAR RP-18e column.

Antibiotic Analysis Tetracyclines Sulfonamides Quinolones

LC-MS/MS-based determination of 19 sulfonamides, 13 quinolones, and 3 tetracycline drug residues in pork meat samples using Supel™ Swift HLB for extraction.

PFAS Column Selection for LC and GC

Guidance on PFAS testing for LC and GC columns, solvent verification, isomer resolution strategies, and hardware choices for trace-level workflows.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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