Anti-phospho GluR4 (Ser842) Antibody

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. GluR4 is an ionotropic glutamate receptor encoded by the GRIA4 gene. Alternative splicings of this gene result in transcript variants encoding different isoforms, which are believed to vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. Direct phosphorylation of GluR4-containing receptors may regulate rapid excitatory synaptic transmission. In particular, phosphorylation of GluR4 at Serine 842 is both necessary and sufficient to deliver the receptor to the synapse and induce plasticity in early postnatal development.

This antibody recognizes GluR4 when phosphorylated at Ser842.

Epitope: Phosphorylated Ser842

Linear peptide corresponding to rat GluR4 phosphorylated at Ser842.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

This Anti-phospho GluR4 (Ser842) Antibody is validated for use in WB for the detection of phospho GluR4 (Ser842).

Evaluated by Western Blot in phosphatase treated and untreated rat cerebellum tissue lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected GluR4 on 10 µg of phosphatase treated and untreated rat cerebellum tissue lysates.

~101 kDa observed. An uncharacterized band may be visible at ~39 kDa in some lysates.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Phosphatase treated and untreated rat cerebellum tissue lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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