25 kDa

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Epitope: Kappa light chain

Prepared from purified mouse IgG light chain.

Suggested dilutions:
Western Blotting: 1:20,000 - 1:400,000 of the 1 mg/mL stock
ELISA: 1:20,000 - 1:400,000 of the 1 mg/mL stock
Immunohistochemistry: 1:500 - 1:5,000 of the 1 mg/mL stock
Immunocytochemistry: 1:500 - 1:5,000 of the 1 mg/mL stock
Flow Cytometry: 1:200 - 1:1,000 of the 1 mg/mL stock

Optimal working dilutions must be determined by the end user.

Detect Mouse light chain using this Goat anti-Mouse light chain Antibody, biotin conjugate validated for use in ELISA, FC, WB, IC, IH(P).

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep immunoglobulins.

ImmunoAffinity Purified

Lyophilized powder from a solution with 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer. Purified by immunoaffinity chromatography.

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.