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Merck
CN

CBL496

Sigma-Aldrich

Anti-CD34 Class II Antibody, clone QBEND/10

clone QBEND/10, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100
300

antibody form

purified antibody

antibody product type

primary antibodies

clone

QBEND/10, monoclonal

species reactivity

monkey, human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

NM_001025109.1
NM_001773.2

UniProt accession no.

P28906

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... CD34(947)

Related Categories

Immunogen

Human endothelial vesicles

Application

Anti-CD34 Antibody, Class II, clone QBEND/10 is an antibody against CD34 Class II for use in FC, IP, IH, IH(P).
Identification of haemopoietic progenitor cells

Studies into the control mechanisms of early haemopoiesis

Purification of haemopoietic cells from, for example, FACS

The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts

Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0.

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

The antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.

KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry.

FUSION PARTNER: NSO myeloma cell line

Physical form

Format: Purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Analysis Note

Tested

Other Notes

Please refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Regulatory Information

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Certificates of Analysis (COA)

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The Fat1 cadherin is overexpressed and an independent prognostic factor for survival in paired diagnosis-relapse samples of precursor B-cell acute lymphoblastic leukemia.
C E de Bock,A Ardjmand,T J Molloy,S M Bone,D Johnstone,D M Campbell,K L Shipman et al.
Leukemia null
Increased microglia/macrophage gene expression in a subset of adult and pediatric astrocytomas.
Engler, JR; Robinson, AE; Smirnov, I; Hodgson, JG; Berger, MS; Gupta, N; James et al.
Testing null
Matthew D Wood et al.
Journal of neuropathology and experimental neurology, 75(10), 925-935 (2016-08-20)
Glioblastomas (GBM) are aggressive brain tumors that inevitably recur despite surgical resection, chemotherapy, and radiation. The degree to which recurrent GBM retains its initial immunophenotype is incompletely understood. We generated tissue microarrays of paired initial and posttreatment GBM (3 pairs
Aisulu Usubalieva et al.
Journal of neuropathology and experimental neurology, 74(10), 960-969 (2015-09-10)
Primary meningeal gliomas are rare tumors composed of a heterogeneous group of neoplasms. We present 2 clinically aggressive cases of primary meningeal pleomorphic xanthoastrocytoma that clinically mimicked meningioma. One case presented in the posterior fossa of a 56-year-old woman; the
L Fina et al.
Blood, 75(12), 2417-2426 (1990-06-15)
All seven of a set of CD34 monoclonal antibodies that recognize epitopes on an approximately 110 Kd glycoprotein on human hemopoietic progenitor cells also bind to vascular endothelium. Capillaries of most tissues are CD34 positive, as are umbilical artery and
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