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Merck
CN

LSKMAGS15

Millipore

PureProteome Magnetic Stand, 15 mL

The PureProteome Magnetic Stand, 15 mL is designed for use with PureProteome Magnetic Beads in affinity purifications (e. g., His-tag purifications or immunoprecipitations).

Synonym(s):

Magnetic Stand for 15 mL Tubes, Magnetic Stand for PureProteome Magnetic Beads

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About This Item

UNSPSC Code:
41116133
eCl@ss:
32011202
NACRES:
NA.56
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material

self-standing

feature

binder

manufacturer/tradename

PureProteome

technique(s)

RNA purification: suitable (with magnetic beads)
protein purification: suitable

shipped in

ambient

General description

PureProteome Magnetic Stand significantly enhance the capture efficiency of PureProteome beads, which possess a high binding capacity.

Application

PureProteome Magnetic Stand, 15 mL I suitable for:
  • purification with magnetic beads
  • protein purification
  • in the purification of the heat shock protein 90 (Hsp90)/ Cdc37 (cell division cycle 37)/ Cyclin-dependent kinase 4 (Cdk4) complex to incubate and wash the beads with 10 bed volumes of lysis buffer

Other Notes

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protein expression and purification of the Hsp90-Cdc37-Cdk4 kinase complex from Saccharomyces cerevisiae
Verba KA and Agard DA
Bio-protocol, 7(19), e2563-e2563 (2017)

Related Content

Read an automated protocol for protein purification using PureProteome™ nickel magnetic beads on the AAW™ automated assay workstation and see results comparing manual vs automated runs.

阅读在 AAW™ 自动测试工作站上使用 PureProteome™ 镍磁珠进行蛋白质纯化的自动化方案,并查看手动与自动运行的结果比较。

Immunoprecipitation (IP) is a powerful technique for proteomic screening, biomarker discovery, and signaling network elucidation. It is frequently used to enrich target proteins from complex samples such as cell lysates or extracts. Traditional IP protocols use Protein A, Protein G or a mixture of Protein A and G coupled to a solid support resin, such as agarose beads, to capture an antigen/antibody complex in solution. As the number of samples increase, the traditional, manual IP method can be time-consuming. Processing of multiple IP reactions in parallel can introduce complexity, variability and pipetting errors, which may affect reproducibility.

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