30.1 kDa calculated

The human B-lymphoblastoid cell line, HRIK.

Epitope: Extracellular domain

Originally reported to stain majority of prolymphocytic leukemia (B-PLL) and hairy-cell leukemia patients derived B cells (Catovsky, D., et al. (1981). Blood. 58(2):406-408.) and later reported to detect a 105 kDa protein species by immunoblotting (Zola, H., et al. (1987). Dis. Markers. 5(4):227-235.), clone FMC7 is now known to target a conformational epitope on the CD20 extracellular region. Clone FMC7 antigenicity is sensitive to levels of membrane cholesterol. Plamsa membrane cholesterol depletion by methyl-β-cyclodextrin treatment results in greatly reduced Ramos surface staining by clone FMC7 (Deans, J.P., and Polyak, M.J. (2008). Blood. 111(4):2492; author reply 2493-4; Polyak, M.J., et al. (2003). Leukemia. 17(7):1384-1389; Serke, S., et al. (2001). Cytometry. 46(2):98-104).

Purified mouse antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD20 in human PBMCs.

ALEXA FLUOR is a trademark of Life Technologies

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.