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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
Quality Level
antibody form
purified antibody
clone
12A11.1
monoclonal
purified by
using protein G
species reactivity
human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
target post-translational modification
unmodified
General description
GST-tagged recombinant protein corresponding to the C-terminal half of human PIF1.
~70 kDa observed. 69.8 kDa (isoform 1), 77.1 kDa (isoform 3), 63.9 kDa (isoform 4) calculated. Uncharacterized band(s) may appear in some lysates.
Specificity
Expected to react with isoforms 1 (isoform alpha) and isoform 4, but not isoform 2. Reactivity toward isoform 3 (isoform beta) has not been determined.
Application
Epitope: C-terminal half
Physical form
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PIF1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PIF1 in 10 µg of HeLa cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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