MABE1874
Anti-RING2/RNF2 Antibody, Clone 3-3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
3-3
monoclonal
purified by
using protein G
species reactivity
mouse, human, human, monkey, mouse, monkey, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
UniProt accession no.
target post-translational modification
unmodified
General description
GST-tagged full length murine RING2 protein.
~42 kDa observed; 37.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated RING2/RNF2 in Immunoprecipitation applications (Yakushiji-Kaminatsui, N., et. al. (2016). Development. 143(2):276-85; Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6; Endoh, M., et. al. (2017). Elife. 6. pii: e27970).
Immunocytochemistry Analysis: A representative lot detected RING2/RNF2 in Immunocytochemistry applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected RING2/RNF2 in Chromatin Immunoprecipitation applications (Farcas, A.M., et. al. (2012). Elife. 1:e00205; Endoh, M., et. al. (2017). Elife. 6. pii: e27970).
Western Blotting Analysis: A representative lot detected RING2/RNF2 in Western Blotting applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).
Immunocytochemistry Analysis: A representative lot detected RING2/RNF2 in Immunocytochemistry applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected RING2/RNF2 in Chromatin Immunoprecipitation applications (Farcas, A.M., et. al. (2012). Elife. 1:e00205; Endoh, M., et. al. (2017). Elife. 6. pii: e27970).
Western Blotting Analysis: A representative lot detected RING2/RNF2 in Western Blotting applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).
Biochem/physiol Actions
Clone 3-3 is a mouse monoclonal antibody that specifically detects E3 ubiquitin-protein ligase RING2 (RING1B).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in U20S cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected RING2/RNF2 in U20S cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected RING2/RNF2 in U20S cell lysate.
Other Notes
Human, Monkey, Mouse.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
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