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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
Quality Level
antibody form
purified antibody
clone
2J3
monoclonal
purified by
using protein G
species reactivity
mouse, human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
General description
Recombinant protein corresponding to human Lin28A.
~26 kDa observed
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28A in A3-1 mouse embryonic stem cells (Narry Kim, Seoul National University).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28A in A3-1 mouse embryonic stem cells (Cho, J., et al. (2012) Cell. 151:765-777).
CLIP-seq Analysis: A representative lot immunoprecipitated Lin28A in A3-1 mouse embryonic stem cells (Cho, J., et al. (2012) Cell. 151:765-777).
Western Blotting Analysis: A representative lot detected Lin28A in A3-1 mouse embryonic stem cells (Narry Kim, Seoul National University).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28A in A3-1 mouse embryonic stem cells (Cho, J., et al. (2012) Cell. 151:765-777).
CLIP-seq Analysis: A representative lot immunoprecipitated Lin28A in A3-1 mouse embryonic stem cells (Cho, J., et al. (2012) Cell. 151:765-777).
Western Blotting Analysis: A representative lot detected Lin28A in A3-1 mouse embryonic stem cells (Narry Kim, Seoul National University).
Physical form
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in NTera2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lin28A in 10 µg of NTera2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lin28A in 10 µg of NTera2 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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