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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
53-6.72, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
technique(s)
flow cytometry: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
mouse ... Cd8A(12525)
General description
T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the CD8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 alpha is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved to form the mature protein that has an extracellular domain (aa 28-196), a helical domain (aa 197-217), and a cytoplasmic tail (aa 218-247). CD8 is generally present as a heterodimer of alpha and beta chains linked by two disulfide bonds, however, it can also form homodimers. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 plays a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 acts as a co-receptor for the T cell receptor (TCR) and like the TCR, it binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets.
Specificity
Clone 53-6.72 is a rat monoclonal antibody that detects CD8 alpha chain in murine thymocytes.
Immunogen
Murine Thymic/splenic lymphocytes.
Application
Flow Cytometry Analysis: A representative lot detected CD8 alpha in Flow Cytometry applications (de Latour, R.P., et. al. (2010). Blood. 116(20):4175-84).
Functional Analysis: A representative lot detected CD8 alpha in Functional analysis applications (Daley, D., et. al. (2016). Cell. 166(6):1485-1499; Hannani, D., et. al. (2014). Cell Death Differ. 21(1):50-8).
Functional Analysis: A representative lot detected CD8 alpha in Functional analysis applications (Daley, D., et. al. (2016). Cell. 166(6):1485-1499; Hannani, D., et. al. (2014). Cell Death Differ. 21(1):50-8).
This rat monoclonal Anti-CD8 alpha, clone 53-6.72, Cat. No. MABF2079, is a rat monoclonal antibody that detects CD8 alpha and has been tested for use in Flow Cytometry and functional studies.
Quality
Evaluated by Flow Cytometry in mouse thymocytes.
Flow Cytometry Analysis: 1 µg of this antibody detected CD8 alpha in one million mouse thymocytes.
Flow Cytometry Analysis: 1 µg of this antibody detected CD8 alpha in one million mouse thymocytes.
Target description
27.46 kDa calculated.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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