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Merck
CN

MABF2198

Sigma-Aldrich

Anti-BCA-225 (BRST-1) Antibody, clone CU-18

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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antibody form

purified antibody

clone

CU18
monoclonal

purified by

using protein G

species reactivity

mouse, human, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

General description

225-250 kDa calculated.
BCA-225 protein secreted by the T47D (Clone 11) human breast carcinoma cell line.

Application

ELISA Analysis: A representative lot detected BCA-225 (BRST-1) in ELISA applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).

Western Blotting Analysis: A representative lot detected BCA-225 (BRST-1) in Western Blotting applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).

Immunocytochemistry Analysis: A representative lot detected BCA-225 (BRST-1) in Immunocytochemistry applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).

Biochem/physiol Actions

Clone CU18 is a mouse monoclonal antibody that detects human breast carcinoma associated glycoprotein BCA-225.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected BCA-225 (BRST-1) in human breast cancer tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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Certificates of Analysis (COA)

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