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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44
biological source
mouse
Quality Level
conjugate
PE-Cy7
antibody form
purified antibody
antibody product type
primary antibodies
clone
104, monoclonal
species reactivity
mouse
concentration
0.2 mg/mL
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Ptprc(19264)
General description
The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.
This mouse monoclonal antibody Anti-CD45.2 (mouse), PE-Cy7, clone 104 validated for use in IP, Flow Cytometry & ICC for the detection of CD45.2.
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 µg of this antibody detected CD45.2 in one million C57Bl/6 splenocytes.
Flow Cytometry Analysis: 0.25 µg of this antibody detected CD45.2 in one million C57Bl/6 splenocytes.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Certificates of Analysis (COA)
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