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MABS1235

Sigma-Aldrich

Anti-Progesterone Receptor A/B Antibody, clone 488/H3

clone 488/H3, from mouse

Synonym(s):

Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, Progesterone Receptor A/B

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

488/H3, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_000917
NP_001189403

UniProt accession no.

P06401

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PGR(5241)

General description

Progesterone receptor (UniProt P06401; also known as nuclear receptor subfamily 3 group C member 3, PR) is encoded by the PGR (also known as NR3C3) gene (Gene ID 5241) in human. The progesterone receptor (PR) is a hormone activated transcription factor, where hormone binding triggers the dissociation of heat shock and chaperone proteins from PR, leading to PR dimerization and activation. The receptor dimer then binds progestin response elements in the regulatory regions of its target genes and a multi-component complex is assembled to enable transcription. In addition, a number of kinases are reported to regulate PR activity via phosphorylation. PR can also regulate transcription without direct DNA binding by tethering to other transcription factors, such as specificity protein 1 (Sp1) or activator protein 1 (AP-1), and modulating their transcriptional activity on target genes. PR is expressed as five isoforms, including PR-B and PR-A that are produced as a result of alternate estrogen inducible promoters within the same PGR gene. PR-A lacks the first 164 amino acids of PR-B, but the two isoforms have otherwise identical sequence. PR-B and PR-A can regulate distinct subsets of target genes and studies in knockout mice suggest that PR-B predominantly regulates mammary gland development and PR-A is critical for normal uterine function.
~110/80 kDa observed

Immunogen

Recombinant protein corresponding to the N-terminal half fragment of human Progesterone Receptor B.

Application

Immunocytochemistry Analysis: A representative lot detected exogenously expressed progesterone receptor isoform A and isoform B (PR-A & PR-B) in transfected MCF10A cells by fluorescent immunocytochemistry (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Western Blotting Analysis: A representative lot detected endogenous progesterone receptor isoforms A & B (PR-A & PR-B) in human breast cancer T47D cell lysate, as well as exogenously expressed PR-A & PR-B in transfected DCIS.COM human ductal carcinoma in situ (DCIS) breast cancer cells, but not in untransfected DCIS.COM cells (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
This Anti-Progesterone Receptor A/B Antibody, clone 488/H3 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor A/B.

Biochem/physiol Actions

Clone 488/H3 reacts with both isoform 1 (isoform B; PR-B) and isoform 2 (isoform A; PR-A). This clone is also expected to react with isoform 5 (delta4), but not isoform 3 or 4 (PR-M).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in human breast cancer T47D cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected both isoforms A and B (PR-A & PR-B) of progesterone receptor in human breast cancer T47D cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Post-Translational Modifications

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