MABT1534
Anti-MARVELD2/Tricellulin Antibody, clone N54
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
N54
monoclonal
purified by
using protein G
species reactivity
mouse, rat, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
target post-translational modification
unmodified
General description
GST-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal region of murine Tricellulin.
~64 kDa observed; 63.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected MARVELD2/Tricellulin in mouse liver and kidney tissue sections.
Western Blotting Analysis: A representative lot detected MARVELD2/Tricellulin in Western Blotting applications (Oda, Y., et. al. (2014). J Cell Sci. 127(Pt 19):4201-12; Ikenouchi, J., et. al. (2005). J Cell Biol. 171(6):939-45).
Immunocytochemistry Analysis: A representative lot detected MARVELD2/Tricellulin in Immunocytochemistry applications (Kim, N.K., et. al. (2015). PLoS One. 10(2):e0116931).
Immunofluorescence Analysis: A representative lot detected MARVELD2/Tricellulin in Immunofluorescence applications (Ikenouchi, J., et. al. (2005). J Cell Biol. 171(6):939-45; Oda, Y., et. al. (2014). J Cell Sci. 127(Pt 19):4201-12; Yokouchi, M., et. al. (2016). Elife. 5. pii: e19593).
Western Blotting Analysis: A representative lot detected MARVELD2/Tricellulin in Western Blotting applications (Oda, Y., et. al. (2014). J Cell Sci. 127(Pt 19):4201-12; Ikenouchi, J., et. al. (2005). J Cell Biol. 171(6):939-45).
Immunocytochemistry Analysis: A representative lot detected MARVELD2/Tricellulin in Immunocytochemistry applications (Kim, N.K., et. al. (2015). PLoS One. 10(2):e0116931).
Immunofluorescence Analysis: A representative lot detected MARVELD2/Tricellulin in Immunofluorescence applications (Ikenouchi, J., et. al. (2005). J Cell Biol. 171(6):939-45; Oda, Y., et. al. (2014). J Cell Sci. 127(Pt 19):4201-12; Yokouchi, M., et. al. (2016). Elife. 5. pii: e19593).
Biochem/physiol Actions
Clone N54 is a rat monoclonal antibody that specifically detects murine Tricellulin. It targets an epitope within the N-terminal cytoplasmic domain.
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in EpH4 cell lystate.
Western Blotting Analysis: 1 µg/mL of this antibody detected MARVELD2/Tricellulin in EpH4 cell lystate.
Western Blotting Analysis: 1 µg/mL of this antibody detected MARVELD2/Tricellulin in EpH4 cell lystate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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