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PIXP01250

Millicell® 24 well standing inserts

pore size 12.0 μm, diam. 12 mm, translucent polycarbonate membrane, Tissue Culture (TC)-treated surface, size 24 wells, sterile

Synonym(s):

cell culture inserts, permeable culture insert, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202
Material:
polystyrene housing, translucent polycarbonate membrane
Pore size:
12.0 μm
Sterility:
γ-irradiated, sterile
Binding type:
Tissue Culture (TC)-treated surface
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Quality Level

400

material

polystyrene housing, translucent polycarbonate membrane

sterility

γ-irradiated, sterile

feature

hydrophilic, 0.1x106 pores/cm2 (pore density)

packaging

pack of 50

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell differentiation: suitable, cell migration: suitable

H

10 mm

diam.

12 mm

size

24 wells

surface area

0.6 cm2

working volume

0.4 mL

color

translucent membrane

matrix

Isopore™

pore size

12.0 μm

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

storage temp.

10-30°C

General description

The 12 µm Millicell® insert is designed for cellular migration and invasion assays, featuring a polycarbonate membrane compatible with various extracellular matrix coatings like laminin and collagen. Its porous structure enables effective migration and invasion studies for cells larger than 10 µm, including induced pluripotent stem cells.

Application

  • Cell migration and invasion
  • Transmigration studies of immune cells
  • Chemotaxis
  • Supernatant separation or filtration
  • Specialized cell generation, e.g. SC-β

Features and Benefits

  • Compatible with multiple extracellular matrix coatings, supporting diverse assay needs.
  • Pores sized to allow migration and invasion of larger cells while facilitating supernatant collection for secretion analysis.
  • Essential tool for generating stem cell-derived pancreatic β cells that respond to glucose with high insulin secretion.
  • Enables detailed study of cellular behavior and molecule secretion during treatments.

Legal Information

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

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Protocols

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

View All Protocols

Global Trade Item Number

SKUGTIN
PIXP0125004053252464676