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PSHT010R5

Millipore

Millicell® 24 well Plate

pore size 0.4 μm, polycarbonate membrane, sterile

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eCl@ss:
32011202
NACRES:
NB.22

material

flat wells
polycarbonate membrane
polystyrene

Quality Level

400

description

Polycarbonate cell culture insert with pore size of 0.4 µm used in a 24-well pate for cell attachment, cell culture, cell differentiation, drug transport & permeability.

sterility

sterile

feature

lid

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

H

26.2 mm

L

128 mm

W

84.8 mm

filter diam.

9.5 mm

filtration area

0.7 cm2

plate size

24 wells

working volume

400 μL

matrix

Isopore™

pore size

0.4 μm pore size

input

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type cardiac stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

Related Categories

General description

Millicell-24 Cell Culture Insert Plate is suitable for cell attachment, cell culture, cell differentiation, drug transport, and permeability. 

Application

Millicell-24 Cell Culture Insert Plate, polycarbonate, 0.4 μm has been used for seeding human leukemia monocytic cells and human colorectal adenocarcinoma cells for differentiation. It has also been used to study drug transport across Caco-2 monolayers.

Features and Benefits

  • It possesses an apical assist and basolateral access for easy pipetting
  • It has feet that elevate the plate above the work surface when disassembled from the feeder tray to prevent monolayer contamination
  • Tear drop-shaped receiver wells to prevent air bubbles 

Other Notes

Automation Compatible: Yes

Legal Information

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Zsolt Sáfár et al.
Journal of pharmaceutical sciences, 107(11), 2742-2747 (2018-07-29)
Breast cancer resistance protein (BCRP) is a point of interest in drug-drug interaction safety testing. Therefore, a consensus probe that can be applied as victim in multiple experimental settings is of great benefit. Identification of candidates has been driven by
Daniel Wai-Hung Ho et al.
Nature communications, 12(1), 3684-3684 (2021-06-19)
Interaction between tumor cells and immune cells in the tumor microenvironment is important in cancer development. Immune cells interact with the tumor cells to shape this process. Here, we use single-cell RNA sequencing analysis to delineate the immune landscape and
Ivan Luzardo-Ocampo et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 146, 111835-111835 (2020-11-02)
Bioactive compounds from food products made from natural ingredients such as corn and common bean could target the NLRP3 inflammasome, protein scaffolds with a key role in the moderation of intestinal inflammation. This research aimed to evaluate the anti-inflammatory effect

Protocols

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

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