11428861001
Roche
Peroxidase (POD), activated
from horseradish
Synonym(s):
POD
biological source
horseradish
Quality Level
form
lyophilized
specific activity
800 units/mg protein (at 25 °C with ABTS and H2O2 as substrate (pH 5.0).)
packaging
pkg of 8 mg (~40 mg lyophilizate)
manufacturer/tradename
Roche
optimum pH
6.0-6.5
shipped in
wet ice
storage temp.
2-8°C
General description
Donor: hydrogen-peroxide oxidoreductase
Reagent for the labeling of water-soluble substances carrying primary amino groups with peroxidase from horseradish (HRP).
Capacity: sufficient for approximately 6 mg IgG.
Reagent for the labeling of water-soluble substances carrying primary amino groups with peroxidase from horseradish (HRP).
Capacity: sufficient for approximately 6 mg IgG.
Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as NAD peroxidase, NADP peroxidase, fattyacid peroxidase and a group of non specific enzymes from various sources. It is present in animals, higher plants and other organisms.
Application
Peroxidase (POD), activated has been used in terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) staining and immunohistochemistry.
The enzyme can be used without pre-activation for labeling water-soluble substances with reactive and accessible primary amino groups (e.g., peptides or proteins) with peroxidase for use in analytical methods. It is particularly suitable for the coupling of antibodies (Ig, Ig Fab, and Ig F(ab′)2 fragments from rabbit, mouse, sheep, and goat) with peroxidase. The resulting conjugate is optimal for use in immunochemical detection systems, for example, ELISA, immunohistochemistry, and immunoblotting procedures.
Biochem/physiol Actions
Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc.
Preparation Note
Dissolving the lyophilizate in 0.5 ml double-dist. Water produces a peroxidase concentration of 16 mg/ml.
Analysis Note
Isoenzymes: >90% isoenzyme C (HPLC)
Purity number: 3.0 - 3.5 (A403/A275)
Purity number: 3.0 - 3.5 (A403/A275)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
动植物源性产品
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