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Merck
CN

16990

Bromelain from pineapple stem

powder, yellow-brown, 2-5 U/mg

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About This Item

CAS Number:
UNSPSC Code:
12352204
EC Number:
253-387-5
MDL number:
EC Number:
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form

powder

specific activity

2-5 U/mg

mol wt

Mr ~28000

color

yellow-brown

storage temp.

−20°C

Other Notes

Cysteine protease. Amino acid sequence; Resolution of β-hydroxy-α-amino acids by the action of proteases on their N-acyl methyl esters
1 U corresponds to the amount of enzyme which releases 1 μmol 4-nitrophenol per minute at pH 4.6 and 25°C (Nα-carbobenzoxy-L-lysine-4-nitrophenyl ester as substrate)


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

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A Ritonja et al.
FEBS letters, 247(2), 419-424 (1989-04-24)
The amino acid sequence of stem bromelain, the major cysteine proteinase from pineapple stem is described. It shows that the enzyme is a member of the papain superfamily of cysteine proteinases, but is not very closely related to any other
R. Chenevert et al.
Canadian Journal of Chemistry, 68, 960-960 (1990)