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Merck
CN

17218

Tomato Juice Broth

NutriSelect® Plus, powder, for yeasts, for aciduric bacteria, pkg of 500 g, suitable for microbiology

Synonym(s):

Lactobacilli media, Yeast cultivation media

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
aciduric bacteria, nonselective for Candida spp., nonselective for Lactobacillus spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., yeasts
Application(s):
food and beverages, microbiology

Key Documents

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sterility

non-sterile

form

powder

Quality Level

200

shelf life

limited shelf life, expiry date on the label

composition

dextrose, 10 g/L , dipotassium phosphate, 0.5 g/L , ferrous sulfate, 0.01 g/L , magnesium sulfate, 0.2 g/L , manganese sulfate, 0.01 g/L , monopotassium phosphate, 0.5 g/L , sodium chloride, 0.01 g/L , tomato juice solids, 20 g/L , yeast extract, 10 g/L

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.7±0.2 (25 °C)

application(s)

food and beverages, microbiology

suitability

aciduric bacteria, nonselective for Candida spp., nonselective for Lactobacillus spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., yeasts

Related Categories

General description

Tomato Juice Broth is specifically designed for the cultivation of yeasts and other aciduric microorganisms. Lactobacilli, which grow poorly on standard culture media, require specialized nutrients for optimal growth. Mickle and Breed demonstrated the effectiveness of tomato juice in selectively inhibiting the growth of non-acidophilic bacteria. Dextrose serves as an additional carbon source, while yeast extract provides nitrogen, carbon, and essential B-complex vitamins. Potassium phosphates function as buffering agents, and the inclusion of several inorganic salts in low concentrations supplies essential ions that, along with yeast extract, support bacterial metabolism.

Application

Tomato Juice Broth is widely used in the food and beverage industry and microbiology research for isolating and cultivating acid-tolerant microorganisms. It is suitable for applications requiring the growth of aciduric bacteria and yeasts, making it ideal for studies related to fermentation and microbial analysis.

Features and Benefits

NutriSelect® Plus is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 41.23 g in 1 litre distilled water. Boil to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM0993
BCCL1564
BCCH6726
BCCG1695
BCCC7418

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Articles

Yeast and Molds in Food and Beverages

Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.

Microbiological Media & Detection

Cost-effective molecular biology method for lactobacilli detection is presented as an alternative to PCR-based methods.

Protocols

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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