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Merck
CN

36408

Bromcresol Purple Broth

suitable for microbiology, NutriSelect® Plus

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606
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sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

beef extract, 3.0 g/L , bromo cresol purple, 0.04 g/L , peptic digest of animal tissue, 10.0 g/L , sodium chloride, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | utilization test: suitable, microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

Quality Level

application(s)

microbiology

suitability

bacteria, nonselective and differential for Enterobacter spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Salmonella spp., nonselective and differential for Shigella spp., nonselective and differential for coliforms

Application

Bromcresol Purple Broth is recommended for studying fermentation of carbohydrates by pure culture

Preparation Note

Suspend 18 g in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes containing inverted Durham`s tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. Cool and aseptically add sterile desired carbohydrate to a final concentration of 0.5 - 1.0 %.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Information

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Sharon Maes et al.
Journal of food protection, 82(2), 262-275 (2019-01-27)
After cleaning and disinfection (C&D), surface contamination can still be present in the production environment of food companies. Microbiological contamination on cleaned surfaces can be transferred to the manufactured food and consequently lead to foodborne illness and early food spoilage.

Articles

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

沙门氏菌污染是全球食源性疾病的第二大成因。控制沙门氏菌的暴发是食品监管机构、餐馆和整个食品工业的一项重要任务。沙门氏菌家族包括2300多种血清型的细菌,但其中有两种类型,即肠炎沙门氏菌和鼠伤寒沙门氏杆菌,约占所有人类感染的一半。大多数沙门氏菌的暴发可以追溯到乳制品、家禽和肉制品,但沙门氏菌几乎可以在任何食物上生长。鸡、鸡蛋及其衍生产品的风险特别高。

有关用于分化和检测大肠杆菌和其他大肠菌群的选择性生长培养基的一篇文章。

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