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Merck
CN

51463

Urea Broth

suitable for microbiology, NutriSelect® Basic

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606

Key Documents

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Product Name

Urea Broth, suitable for microbiology, NutriSelect® Basic

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 9.5 g/L
phenol red, 0.01 g/L
potassium dihydrogen phosphate, 9.1 g/L
urea, 20 g/L
yeast extract, 0.1 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

nonselective and identification for Enterobacter spp.
nonselective and identification for Escherichia coli
nonselective and identification for Klebsiella spp.
nonselective and identification for Proteus spp.
nonselective and identification for Salmonella spp.
nonselective and identification for coliforms
nonselective and identification for enterobacteriaceae

Quality Level

100

Related Categories

Application

Differential medium for the detection of urea-utilizing microorganisms, acc. to Rustigian and Stuart (1941).

Other Notes

Adaption of urea broth for radiosterilization
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Dissolve 38.7 g in 1 litre distilled water and filter sterilize. This medium must NOT be heated.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN0470
BCCM0944
BCCJ5477
BCCK8700
BCCG8470

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W.W. Feguson et al.
The Journal of Laboratory and Clinical Medicine, 28, 1715-1715 (1943)
R. Rustigian et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 47, 108-108 (1941)
E Eisenberg et al.
The Journal of applied bacteriology, 58(1), 21-25 (1985-01-01)
Urea broth, a medium used for the identification of the genus Proteus, was sterilized by gamma radiation, using radiation doses of 1-1.5 Mrad. The radiation-sterilized medium, modified by adding sodium ascorbate and increasing its phenol red and yeast extract content

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