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Merck
CN

68943

Millipore

Lee′s Multi-Differential Agar

suitable for microbiology

Synonym(s):

LMDA

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

Key Documents

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shelf life

limited shelf life, expiry date on the label

Quality Level

100

composition

Agar, 15.0 g/L
Bromocresol Green free acid, 0.022 g/L
Calcium carbonate, 5.0 g/L
Calcium pantothenate, 2.0 g/L
Citric acid, 1.10 g/L
Dextrose, 10.0 g/L
Dipotassium phosphate, 0.5 g/L
Ferrous sulfate, 0.01 g/L
Magnesium sulfate, 0.2 g/L
Manganese sulfate, 0.01 g/L
Monopotassium phosphate, 0.5 g/L
Peptonized Milk, 20.0 g/L
Sodium chloride, 0.01 g/L
Tomato Juice Broth, 20.0 g/L
Tween® 80, 0.5 g/L
Yeast extract, 10.0 g/L

final pH

5.5±0.2 (25 °C)

application(s)

microbiology

Application

LMDA (Lee′s Multi-Differential Agar) is a differential solid medium for the detection and enumeration of a variety of brewery bacteria and yeast.

Preparation Note

Mix 84.8 grams of the medium in 1 liter of purified water until evenly dispersed. Heat with repeated stirring until boiling to fully dissolve. Autoclave at 121°C for 15 minutes. Cool medium to approx. 45-50°C in a water bath. Gently and thoroughly swirl the medium, without agitation, in order to uniformly suspend the calcium carbonate precipitate. Dispense the medium into sterile petri dishes.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8949
BCCJ3483

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Protocols

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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