shelf life
limited shelf life, expiry date on the label
Quality Segment
composition
Agar, 15.0 g/L , Bromocresol Green free acid, 0.022 g/L , Calcium carbonate, 5.0 g/L , Calcium pantothenate, 2.0 g/L , Citric acid, 1.10 g/L , Dextrose, 10.0 g/L , Dipotassium phosphate, 0.5 g/L , Ferrous sulfate, 0.01 g/L , Magnesium sulfate, 0.2 g/L , Manganese sulfate, 0.01 g/L , Monopotassium phosphate, 0.5 g/L , Peptonized Milk, 20.0 g/L , Sodium chloride, 0.01 g/L , Tomato Juice Broth, 20.0 g/L , Tween® 80, 0.5 g/L , Yeast extract, 10.0 g/L
final pH
5.5±0.2 (25 °C)
application(s)
microbiology
Application
LMDA (Lee′s Multi-Differential Agar) is a differential solid medium for the detection and enumeration of a variety of brewery bacteria and yeast.
Preparation Note
Mix 84.8 grams of the medium in 1 liter of purified water until evenly dispersed. Heat with repeated stirring until boiling to fully dissolve. Autoclave at 121°C for 15 minutes. Cool medium to approx. 45-50°C in a water bath. Gently and thoroughly swirl the medium, without agitation, in order to uniformly suspend the calcium carbonate precipitate. Dispense the medium into sterile petri dishes.
Legal Information
TWEEN is a registered trademark of Croda International PLC
存储类别
11 - Combustible Solids