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Merck
CN

93309

TRIS borate – EDTA buffer substance

for Northern and Southern blotting, powder

Synonym(s):

TBE buffer dry blend

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105319

Key Documents

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grade

Molecular Biology

product line

BioReagent

form

powder

quality

5× concentrate

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

color

white

pH

8.1-8.5

storage temp.

room temp

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble and protected from degradation. This powder should be reconstituted in molecular biology grade water before use.

Application

TRIS borate – EDTA buffer substance is suitable for use as running buffer for agarose gel electrophoresis and nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Other Notes

The final 5x TBE liquid contains 0.445M TRIS borate (pH 8.3) and 0.01M EDTA in water.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360FD

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBZ6338
BCBW9090
BCBW7764
BCBT7110
BCBS6677V

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Detection of Chicken Meat Adulteration in Raw Hamburger Using
Polymerase Chain Reaction
M. Mehdizadeh
Journal of Food Quality (2014)

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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