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Merck
CN

93309

Sigma-Aldrich

TRIS borate – EDTA buffer substance

for Northern and Southern blotting, powder

Synonym(s):

TBE buffer dry blend

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

Key Documents

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grade

Molecular Biology

product line

BioReagent

form

powder

quality

5× concentrate

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

color

white

pH

8.1-8.5

storage temp.

room temp

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble and protected from degradation. This powder should be reconstituted in molecular biology grade water before use.

Application

TRIS borate – EDTA buffer substance is suitable for use as running buffer for agarose gel electrophoresis and nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Other Notes

The final 5x TBE liquid contains 0.445M TRIS borate (pH 8.3) and 0.01M EDTA in water.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H360FD

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBZ6338
BCBW9090
BCBW7764
BCBT7110
BCBS6677V

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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