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Merck
CN

94742

Vanadyl ribonucleoside complexes solution

BioReagent, Molecular Biology, 200 mM

Synonym(s):

Ribonucleoside vanadyl complexes

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About This Item

NACRES:
NA.52
UNSPSC Code:
12352200
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Product Name

Vanadyl ribonucleoside complexes solution, BioReagent, Molecular Biology, 200 mM

Quality Level

grade

Molecular Biology

product line

BioReagent

form

liquid

concentration

200 mM

impurities

oxovanadium V

suitability

in accordance for ribonuclease inhibition test

shipped in

dry ice

storage temp.

−20°C

Application

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes solution has been used:
  • as a component of polysome lysis buffer and fluorescence in situ hybridization (FISH) buffer
  • as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes

General description

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.
Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase). Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Regulation of m 6 A Transcripts by the 3?? 5? RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline
Wojtas MN, et al.
Molecular Cell, 68(2), 374-387 (2017)
Immunoprecipitation of mRNA-protein complexes
Peritz T, et al.
Nature Protocols, 1(2), 577-577 (2006)
Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments
Del Prete MJ, et al.
RNA, 13(3), 414-421 (2007)
P A Takizawa et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(10), 5273-5278 (2000-05-03)
In Saccharomyces cerevisiae, mRNA encoding the cell-fate determinant Ash1p is localized to the distal tip of daughter cells. Five SHE genes are required for proper Ash1 mRNA localization, one of which encodes the myosin Myo4p. We show that three of
Wei Wang et al.
Nucleic acids research, 36(15), 4913-4928 (2008-07-26)
The mechanisms of influenza A virus mRNA intracellular transport are still not clearly understood. Here, we visualized the distribution and transport of influenza A virus mRNA in living cells using molecular beacon (MB) technology. Confocal-FRAP measurements determined that the transport

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