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T5574

Terrific Broth

Liquid microbial growth medium

Synonym(s):

TB medium, Tartoff – Hobbs medium

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About This Item

NACRES:
NA.85
UNSPSC Code:
41106200
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Quality Level

500

grade

Molecular Biology

description

Liquid Medium

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Terrific Broth has been used for non-selective cultivation of E. coli strains for cloning, high-yield DNA plasmid production, and high-yield production of recombinant proteins. citation It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Terrific Broth liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Other Notes

23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

动物来源培养基

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Certificates of Analysis (COA)

Lot/Batch Number
0000407886
0000407886
0000408192
0000408192
SLCN1739

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Protocols

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Shani Leviatan et al.
The Journal of biological chemistry, 285(31), 23548-23556 (2010-06-08)
Membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for
The preparation and analysis of DNA for use in transgenic technology.
Auerbach, A.B., et al.
Advanced Protocols For Animal Transgenesis, 501-501 (2011)
Shaheen Shojania et al.
Journal of virological methods, 164(1-2), 35-42 (2009-11-28)
The HIV-1 transactivator of transcription (Tat) is a protein essential for virus replication. Tat is an intrinsically disordered RNA-binding protein that, in cooperation with host cell factors cyclin T1 and cyclin-dependent kinase 9, regulates transcription at the level of elongation.
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Global Trade Item Number

SKUGTIN
T5574-500ML04061837347993
CR40057-1000M04061838445797