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22Rv1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5092802, human prostate, Epithelial

Synonym(s):

22Rv-1 Cells, CWR-22rv1 Cells, CWR22-R1 Cells

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About This Item

UNSPSC Code:
41106514
Biological source:
human prostate
Relevant disease(s):
cancer
Growth mode:
Adherent aggregates
Karyotype:
49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+12[5]/50,idem,+3[1]
Morphology:
Epithelial

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biological source

human prostate

packaging

tube of 5 μg 05092802-DNA-5UG, pkg of vial of cells 05092802-1VL

growth mode

Adherent aggregates

karyotype

49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+12[5]/50,idem,+3[1]

morphology

Epithelial

products

Not specified

receptors

androgen receptor positive

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Biochem/physiol Actions

22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor β-1 (TGF β-1). This cell line is tumorigenic in nude mice.
Human Xenograft Prostate
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 15,21

Preparation Note

RPMI 1640 without phenol red (Sigma R7509) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Regulatory Information

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