KARPAS 299
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Synonym(s):
K299 Cells, KARPAS299 Cells
biological source
human lymph
packaging
tube of 5 μg 06072604-DNA-5UG
pkg of vial of cells 06072604-1VL
growth mode
Suspension
karyotype
Hypodiploid
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin′s lymphoma in 1986.
Human Non-Hodgkin′s Ki-positive Large Cell Lymphoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19
Preparation Note
Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Regulatory Information
动植物来源培养基
高风险级别生物产品--人源产品
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