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About This Item
UNSPSC Code:
41106514
Biological source:
human embryo (carcinoma)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Not specified
biological source
human embryo (carcinoma)
packaging
tube of 5 μg 06072611-DNA-5UG, pkg of vial of cells 06072611-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A human embryonal carcinoma cell line derived from a human testicular germ cell tumour.
Human embryonal carcinoma
Preparation Note
DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.
Split sub-confluent cultures (70-80%), approximately every 3-4 days, 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. Cells need to be maintained at a high cell density.
Other Notes
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