N1E-115-1

can be used as a neuronal model

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

Mouse neuroblastoma

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.