biological source
human blood
packaging
tube of 5 μg 11060101-DNA-5UG, pkg of vial of cells 11060101-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Small rounded cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
-140 to -196°C
Biochem/physiol Actions
Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific for non-structural protein NS3 (helicase domain). This antibody binds to a major conformational epitiope located at the carboxyl-terminal end of the NS3 region that is recognised by approximately 90% from HCV-infected patients. CM3.B6 can be used in immunochemistry, HCV protein purification and HCV typing. CM3.B6 and B12.F8 (Sigma product number 11060102) were derived from the same patient as confirmed by STR profiling.
Human anti-hepatitis C virus IgG1 secreting B cell
STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16
Preparation Note
RPMI 1640 (R0883) + 2 mM L-L-Glutamine (G7513) + 1% NEAA (M7145) + 1% NaP (S8636) + 10% FBS / FCS (F2442) + 20U/ml Interlukin-6 (IL-6).
Maintain cultures between 3-9 x 105/ml. 5% CO2; 37 °C. Media change every two days. Saturation density 1.1 x 106/ml
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by any organization may require a license agreement. Customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.