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About This Item
UNSPSC Code:
41106514
Biological source:
human blood
Growth mode:
Suspension
Karyotype:
Not specified
Morphology:
Small rounded cells
biological source
human blood
packaging
tube of 5 μg 11060102-DNA-5UG, pkg of vial of cells 11060102-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Small rounded cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
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Biochem/physiol Actions
Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific to the native HCV nucleoprotein N3. This antibody binds to native HCV nucleoprotein with no observable reactivity for the NS3, NS4 and NS5 regions. This antibody identifies a major B cell epitope within the immunodominant nucleoprotein amino terminal subregion (aa. 34-45). B12.F8 can be used in immunochemistry, HCV protein purification and HCV typing.CM3.B6 (Sigma product number 11060101) and B12.F8 were derived from the same patient as confirmed by STR profiling.
Human anti-hepatitis C virus IgG1 secreting B cell
STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16
Preparation Note
RPMI 1640 (R0883) + 2 mM L-L-Glutamine (G7513) + 1% NEAA (M7145) + 1% NaP (S8636) + 10% FBS / FCS (F2442) + 20U/ml Interleukin-6 (IL-6).
Maintain cultures between 3-9 x 105/ml. 5% CO2; 37 °C. Media change every four days. Saturation density 1.1 x 106/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
Regulatory Information
常规特殊物品
低风险生物材料
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