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111A3

Name: 111A3
Brand: SIGMA

85062504, Application: Fusion partner in the production of heterohybridomas, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., no DNA

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About This Item

UNSPSC Code:

41106514

Biological source:

Mouse x Bovine Heterohybridoma

Isotype:

Not specified

Technique(s):

cell culture | mammalian: suitable

Myeloma:

53B3 (mouse x bovine heteromyeloma)

Reactivity:

Application: Fusion partner in the production of heterohybridomas

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Properties

biological source

Mouse x Bovine Heterohybridoma

packaging

tube of 5 μg 85062504-DNA-5UG, pkg of vial of cells 85062504-1VL

ImmunoDon

Jersey calf lymph node

Myeloma

53B3 (mouse x bovine heteromyeloma)

Reactivity

Application: Fusion partner in the production of heterohybridomas

technique(s)

cell culture | mammalian: suitable

isotype

Not specified

shipped in

dry ice

storage temp.

−196°C

Description

Immunogen

Sheep red blood cells

Application

This line has been used as a HAT sensitive fusion partner in the production of heterohybridomas

Biochem/physiol Actions

Mouse 53B3 x Bovine Jersey Calf lymph node

Non Ig secreting

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.

Regulatory Information

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