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BW5147.G.1.4.OUA/R.1

85082302

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biological source

mouse blood

growth mode

Suspension

morphology

Lymphoblast

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

For complete product information, please see BW5147.G.1.4.OUA/R.1

Cell Line Origin

Mouse AKR/J T cell lymphoma

Cell Line Description

This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

Application

Fusion partner

Culture Medium

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 1-6x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

新产品

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Articles

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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