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About This Item
UNSPSC Code:
41106514
Biological source:
human muscle
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Near Diploid
Morphology:
Epithelial-like
biological source
human muscle
packaging
tube of 5 μg 85111504-DNA-5UG, pkg of vial of cells 85111504-1VL
growth mode
Adherent
karyotype
Near Diploid
morphology
Epithelial-like
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
products
Transforming growth factor (Tramnsforming Growth Factor (TGF)) - β
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.
The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.
The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.
Human Ewing sarcoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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