AFRC MAC 74
Reactivity: Anti-Nitrate Reductase, 86112690, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 86112690-DNA-5UG
pkg of vial of cells 86112690-1VL
ImmunoDon
F344 rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Nitrate Reductase
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
Nitrate reductase purified from the leaves of 6-8 week old spinach plants - Spinacea oleraceal
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat F344 spleen
The antibody is specific for NADH nitrite reductase and inhibits reduction of nitrate to nitrate using NADH electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) or methyl viologen. The antibody is directed against NADH-NR if mono and dicotyledons.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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