AFRC MAC 79
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-Nitrate Reductase, 86112691
biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 86112691-DNA-5UG
pkg of vial of cells 86112691-1VL
ImmunoDon
F344 rat spleen
Myeloma
YB2/3.0 Ag20
Reactivity
Anti-Nitrate Reductase
technique(s)
cell culture | mammalian: suitable
isotype
IgG
shipped in
dry ice
storage temp.
−196°C
Immunogen
Nitrate reductase purified from the leaves of 6-8 week old spinach plants - Spinacea deracea
Application
Affinity purification of nitrate reductase
Biochem/physiol Actions
Rat YB2/3.0 Ag20 x Rat F344 spleen
The antibody is specific for NADH-nitrate reductase of spinach and marrow. It inhibits the reduction of nitrate to nitrite using NADH as elector donor and also inhibits dehydrogenase activity of NADH - Cytochrome c reductase.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse feeder layers upon resuscitation
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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