CRI-G1

Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon.

Not specified

Rat NEDH islet tumour

DMEM + 2 mM Glutamine + 5-10% Fetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. The cells tend to grow in clumps and ribbons. They do not strictly attach but stick lightly and will not form a complete monolayer.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.