COLO 205 Cell Line human

Isolated from ascitic fluid of a 70 year old Caucasian male with carcinoma of the colon. Produces carcinoembryonic antigen.

tumourigenic in suitable animals

COLO 205, COLO 201 (Sigma product no. 87091201) and COLO 206 (Sigma product no. 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

Human Caucasian colon adenocarcinoma

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split subconfluent cultures of attached cells 1:3 to 1:6 seeding at 2-3x10⁴ cells/cm²; 5% CO₂; 37°C.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.