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About This Item
UNSPSC Code:
41106514
Biological source:
hamster ovary
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
hamster ovary
packaging
tube of 5 μg 89030316-DNA-5UG, pkg of vial of cells 89030316-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Ornithine decarboxylase
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Enzymatic studies
Biochem/physiol Actions
Hamster Chinese ovary
Overproduces ornithine decarboxylase in response to polyamine deprivation.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + Ham′s F12 (without Putrescine) (1:1) + 0.1% BSA.
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 minutes or until cells detach. Culture cells at 37°C; 5% CO2. Pre-soak flasks in porcine
Other Notes
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