DBS-FRhL-2

Monkey Rhesus lung

A diploid cell line derived from normal male Rhesus monkey fetus. Susceptible to poliovirus types 1-3, Coxsackie A9, parainfluenza 3, rubella and is non-tumourigenic in animals.

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

Split sub-confluent cultures (70-80%) 1:2 to 1:3, i.e., seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37 °C. Finite lifespan. Cell line has been shown to undergo 51 passages or 74 population doublings.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 4,040,905.