1306
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human skin
packaging
tube of 5 μg 90011887-DNA-5UG
pkg of vial of cells 90011887-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Transfection studies
Biochem/physiol Actions
A HPRT- derivative of the SV40 transformed human fibroblast line GM0637. The cells were selected in 5μg/ml thioguanine and are useful for transfection experiments.
Human skin fibroblast
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 13
D16S539: 12,13
D5S818: 11
D7S820: 10
THO1: 7,9
TPOX: 9,11
vWA: 16,17
CSF1PO: 11,12
D13S317: 13
D16S539: 12,13
D5S818: 11
D7S820: 10
THO1: 7,9
TPOX: 9,11
vWA: 16,17
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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