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About This Item
UNSPSC Code:
41106514
Biological source:
rat liver
Growth mode:
Adherent
Karyotype:
Diploid
Morphology:
Epithelial
biological source
rat liver
packaging
tube of 5 μg 90071806-DNA-5UG, pkg of vial of cells 90071806-1VL
growth mode
Adherent
karyotype
Diploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Transformation studies
Biochem/physiol Actions
A spontaneous transformant of the cell line ARLJ 301-3 from the F334/Ducrj rat.
Rat F334/Ducrj liver
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
William′s E + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Regulatory Information
动物来源培养基
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