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Merck
CN

MC116

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
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biological source

human lymph

packaging

tube of 5 μg 90110501-DNA-5UG
pkg of vial of cells 90110501-1VL

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Surface Immunoglobulin M (IgM), pentameric Immunoglobulin M (IgM)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Hormone and tumourigenicity studies

Biochem/physiol Actions

Derived from the pleural effusion of a patient with undifferentiated lymphoma. The cells are EBNA negative, express surface IgM Lambda and secrete pentameric IgM which does not bind to protein A. Complement and Fc receptors were not detected.
Human lymphoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9.3,10
TPOX: 8,10
vWA: 15,17

Preparation Note

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37°C. A maximum cell density of 2-4x1,000,000 cells/ml is achieved.
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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