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About This Item
UNSPSC Code:
41106514
Biological source:
human kidney
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, aneuploid
Morphology:
Epithelial
biological source
human kidney
packaging
tube of 5 μg 93020513-DNA-5UG, pkg of vial of cells 93020513-1VL
growth mode
Adherent
karyotype
2n = 46, aneuploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from 78-year-old male. Does not produce tumours in nude mice.
Human kidney adenocarcinoma
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Regulatory Information
低风险生物材料
常规特殊物品
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