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Merck
CN

A.704

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93020513, human kidney, Epithelial

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About This Item

UNSPSC Code:
41106514
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biological source

human kidney

packaging

tube of 5 μg 93020513-DNA-5UG
pkg of vial of cells 93020513-1VL

growth mode

Adherent

karyotype

2n = 46, aneuploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Tumourigenicity studies

Biochem/physiol Actions

Derived from 78-year-old male. Does not produce tumours in nude mice.
Human kidney adenocarcinoma

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

动植物来源培养基
高风险级别生物产品--人源产品
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